ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing
Ted Wong, Ira W Deveson, Simon A Hardwick, Tim R Mercer, ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing, Bioinformatics, Volume 33, Issue 11, June 2017, Pages 1723–1724, https://doi.org/10.1093/bioinformatics/btx038
Abstract
Summary
Spike-in controls are synthetic nucleic-acid sequences that are added to a user’s sample and constitute internal standards for subsequent steps in the next generation sequencing workflow.
The Anaquin software toolkit can be used to analyze the performance of spike-in controls at multiple steps during RNA sequencing or genome sequencing analysis, providing useful diagnostic statistics, data visualization and sample normalization.

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