Technology

Mirroring Technology & Innovative Design

Maximize data insights across a range of applications, collaborations and locations with simple to use sequencing spike-in controls

Mirroring Technology & Innovative Design
Mirroring Technology & Innovative Design
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Sequins™ are synthetic nucleic acid controls that directly mirror naturally occurring sequences

Because Sequins retain the same nucleotide composition as the natural sequence, they enable accurate representation of genomic complexity without compromising integrity of the sample and results. Sequins perform equivalently throughout sequencing workflows, providing a true measure of control.

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Simple Workflow That Leverages Established Pipelines

01

Target sequences

Identify specific sequences of interest to begin the process with precision.
02

Produce mirrored Sequins

Generate Sequins that mimic the behavior of target sequences, ensuring accurate calibration.
03

Spike Sequins into samples

Sequins are spiked into each sample before library preparation and processed alongside the sample through the sequencing workflow.
04

Prepare libraries and sequence

Libraries are prepared, and sequencing is performed to capture both sample- and Sequins-derived reads.
05

Analyze combined FASTA files

A Sequins-specific FASTA file is provided for simple concatenation with the reference genome, enabling seamless analysis.
06

Sequins act as internal controls

Sequins-derived reads are calibrated and analyzed in parallel with sample-derived reads using standard bioinformatics software.
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Best In-Class Normalization & Quantification

Log2 Kmer Count
Read Count Analysis

Log2 kmer count comparison between two genomes (A & B) before and after Sequins normalization.

Adapted from Reis et al (2020)

Read count before and after Sequins normalization for whole genome (blue) and gene panel (orange) sequencing.

Adapted from Reis et al (2020)

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