Products & Applications

Sample Tracking Control Set v1.0

Internal spike-in controls for sample tracking, carry-over assessment and workflow integrity.

Sample Tracking Control Set v1.0
Sample Tracking Control Set v1.0
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Cross-sample contamination, carry-over and sample swaps negatively impact operational efficiency and cost of sequencing workflows.
The Sequins Sample Tracking Control Set v1.0 comprises combinations of mirrored, non-homologous molecules that are spiked into each sample prior to sequencing.
Enables sample tracking, swap detection, carry-over monitoring and workflow QC insights.
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Sequins (sequencing spike-ins) are synthetic nucleic acid controls that mirror naturally occurring sequences while remaining analytically distinguishable from the native sample. The mirrored Sequins design enables control-derived reads to be detected separately from sample-derived reads in the sequencing output, allowing the Sequins controls to be assessed without interfering with interpretation of sample data.

 

Building on this core Sequins technology, the Sample Tracking Control Set v1.0 provides an in-sample, sequencing-compatible molecular tracking system for 96-well plate-based workflows. Each sample is assigned to a planned well, spiked with a predefined well-specific combination of three Sequins molecules, processed through the workflow and assessed after sequencing. Together, the three Sequins molecules form a defined molecular signature that links each sample to its expected well position.

Sample Tracking Control Set

Workflow for using well-specific Sequins molecule combinations as in-sample molecular tracking controls. A predefined well-specific combination of three Sequins molecules creates a unique set of molecular signatures. These predefined combinations are added to each sample at a selected workflow stage, processed with the sample, and detected bioinformatically after sequencing to compare the observed Sequins signal with the planned plate layout.

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Control Set uses

Sample tracking and workflow QC

The Sample Tracking Control Set v1.0 can support two complementary applications: (1) internal, sample-specific molecular tracking, and (2) workflow QC review based on recovery of known Sequins inputs.

Sample tracking

Compare observed Sequins signatures with planned well assignments from the point of addition.

Carry-over monitoring

Unexpected Sequins molecules assigned to another well may indicate carry-over or cross-sample contamination.

Sample swap detection

A discordant signature may indicate a possible sample-to-well assignment issue or sample swap.

Workflow QC insights

Recovery of expected Sequins molecules can support sample-level and run-level QC review.

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